All the dogs, cats, puppies and kittens heading to Chicago were in shelters in the Naples and Fort Meyers areas before Hurricane Ian hit.
They are being moved so that animals who got lost during the hurricane will have someplace to stay.
The volunteers and pets are expected to arrive at PAWS Chicago on Tuesday afternoon.
The pets will be given medical care, vaccinations, and microchips as needed. Once they are cleared, they will be ready for adoption.